G.L.A.D.I.S. (Gadget Lending And Distribution Interactive System) was introduced in the episode "Physics 101 Much?" of Season 3, as a Gadget lending device. She speaks with a female voice and appears to have a mind of her own, even sometimes claiming she's the true leader of WOOHP. In several episodes she ignores Jerry's orders and distributes the gadgets she believes are needed for the mission.
It's later revealed in "Evil G.L.A.D.I.S. Much?" that her brain is downloaded from a brilliant evil mastermind called the Brain who attempted to start World War 3 by misleading nations into firing missiles at one another but did not have any of his evil downloaded into her. Alexandra accidentally spilled cranberry juice on her and she became evil because she shares the same allergy as the Brain, which results in both being crazy after being spilled with cranberry juice. The only way that G.L.A.D.I.S. could turn back to normal was by having her mainframe washed with a base to counteract the acid in the cranberry juice.
In "Evil Roommate", the second episode of Season 5, Jerry Lewis sent her to the WOOHP recycling facility because of her attitude, claiming that her personality had landed her an extended "vacation". Jerry begins distributing the gadgets for the girls again. Judging by Jerry's tone of voice, he had G.L.A.D.I.S. dismantled.
- G.L.A.D.I.S. bears a striking resemblance (in name and personality) to GLaDOS from the videogame "Portal". However, "Portal" was released in 2007, 3 years after her first appearance in "Physics 101 Much?".
- She also resembles the character Shirka, from the French-Japanese animation "Ulysses 31".
- There are some similiarities with HAL 9000 from the Space Odyssey series.
- In "Power Yoga Much?" G.L.A.D.I.S. gains a new hobby: magic tricks. When she hands out gadgets to the spies in the episode, she does so as if performing various introductory level magic tricks such a pulling a rabbit out of a hat; but instead of a rabbit she pulls out a Black Belt and other gadgets.
- In "Mime Your Own Business" it is said that G.L.A.D.I.S was in love with Jerry's photocopier.
- She was written out of the series almost entirely due to fan complaints.